Effect of genotype on androgenesis in barley (Hordeum vulgare L.)

Current study was set up to determine the response of 12 Iranian winter diploid barley (Hordeum vulgare L.) cultivars to anther culture on solid FHG induction medium supplemented with 90 mg/l sucrose, 2 mg/l 2,4-D and 0.1 mg/l kinetin. For cold pretreatment each selected spike was kept in a refrigerator at 4 C for 14 days. The experimental design consisted of a completely randomized design (CRD) with four replications. Embryogenesis and green plant regeneration occurred in all genotypes. The number of embryoids and total plant regeneration as well as the numbers of green and albino plants regenerated per 100 anthers, was recorded. The results showed that genotype affected significantly on embryo induction, total plant regeneration, green and albino regeneration. Genotype No. 9 gives the best results for embryo production, green and total plant regeneration (74.792%, 7.293% and 17.29%, respectively). Linear correlation analysis showed a positive and significant (p<0.01) relationship between embryo induction and green plant regeneration.